DNA replication in adult and aged rat tissues
Levitskiĭ, E.L.
Biokhimiia 47(10): 1601-1607
1982
ISSN/ISBN: 0320-9725 PMID: 7171644 Document Number: 186315
The incorporation of single-stranded DNA replication intermediates (Okazaki fragments) into double-strand polymerized DNA and the chain growth of these intermediates in adult and aged rat tissues with low and high mitotic activities were studied. These processes were found to proceed in a similar manner independently of the tissue mitotic activity. However, in tissues with a low mitotic activity of adult and aged rats the incorporation of Okazaki fragments into double-stranded DNA was decelerated as compared to those with a high mitotic activity. Since the mitotic activity and ageing were shown to have similar effects on the rate of stabilization of replicating DNA secondary structure, it may be assumed that the factors responsible for regulation of DNA replication rate are altered with ageing.