Mouse hepatitis virus immunofluorescence in formalin- or Bouin&apos;s-fixed tissues using trypsin digestion

Brownstein, D.G.; Barthold, S.W.; Brownstein, D.G; Barthold, S.W

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Mouse hepatitis virus immunofluorescence in formalin- or Bouin's-fixed tissues using trypsin digestion

Brownstein, D.G.; Barthold, S.W.

Laboratory Animal Science 32(1): 37-39

1982

ISSN/ISBN: 0023-6764

PMID: 6281569

Document Number: 184977

Mouse hepatitis viral antigens were demonstrated by immunofluorescence in formalin- and Bouin's-fixed tissues processed routinely for histopathological studies with partial digestion by trypsin. Staining was best in tissues fixed in formalin and was not diminished in tissue sections from paraffin blocks stored at room temperature as long as two years. The relative ease of this procedure and the commercial availability of reagents makes this a useful technique for the definitive diagnosis of mouse hepatitis virus infection.