Molecular pathology and clinicobiological correlations of IgD myeloma
San Miguel, J.; Hernández, F.; Vicente, V.; Lasierra, J.; Sánchez, S.; López Borrasca, A.
Medicina Clinica 78(1): 1-4
1982
ISSN/ISBN: 0025-7753 PMID: 7062796 Document Number: 184559
Four patients with a confirmed diagnosis of IgD myeloma were studied. All presented .lambda. Bence Jones proteinuria and the high numbers of plasma cells associated with an extremely poor prognosis (1.36, 1.21, 1.15 .cntdot. 1012 cells/m2 body surface). Light chain peptidemia was seen in three, amyloidosis in one and normocalcemia, renal insufficiency and anemia in 2 patients in clinical state III B and two in clinical state II A. Sera were separated on cellogel R.S. strips, eluted and identified by double diffusion and both whole sera and the eluates containing the paraprotein precipitated by bi-dimensional electrophoresis. Samples were first stained with CNOK. This technique showed the separation of 1 M components into 2 fragments of different mobility and confirmed the lability of IgD, which, through trypsin activity, separates into Fc and Fab fragments and, after incubation, into VHC.lambda. fragments. Osteosclerotic lesions were found in 1 patient.