Reactivation of DNA replication of the parvovirus MVM in mouse cells preexposed to UV radiation
Vos, J.M.; Rommelaere, J.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 176(3): 395-401
1982
ISSN/ISBN: 0037-9026 PMID: 6215105 Document Number: 183651
The parvovirus Minute-Virus-of-Mice (MVM) was used to probe the DNA replication activities expressed by mouse fibroblasts. This system allowed us to study quantitatively the effect of UV-induced DNA lesions on the progression of DNA replication in vivo. MVM was UV-irradiated prior to infection. Pyrimidine dimers induced in the viral genome account for the reduced level of intracellular viral DNA synthesis, assuming that most of these lesions block viral DNA replication in unirradiated cells. The inhibition of damaged MVM DNA synthesis is less severe if the host cells themselves are irradiated prior to virus infection. This stimulation of viral DNA replication in pretreated cells might account for the UV-enhanced viral reactivation phenomenon, i.e. the increased survival of nuclear-replicating viruses propagated in cells preexposed to various genotoxic agents.