Leukocyte migration inhibition in chickens inoculated with Salmonella typhimurium
Nagaraja, K.V.; Newman, J.A.; Pomeroy, B.S.
American Journal of Veterinary Research 43(5): 916-918
1982
ISSN/ISBN: 0002-9645 PMID: 7046540 Document Number: 183420
Leukocyte migration-inhibition tests were conducted in chickens infected with Salmonella typhimurium. A relatively new and simple method that involves the migration of leukocytes under agarose gel was used. Migration distance was projected onto a white background at 40X magnification, using a microprojector. Quantitation of migration was done by measuring the linear distance the cells had moved from the margin of the agarose wells. The details of the technique and the importance of it in studies of cell-mediated immune response are discussed. Chickens inoculated with S typhimurium had a significant leukocyte migration inhibition.