Liquid storage media for vascular prosthesis of biological origins : their impact on biocompatibility
Sigot-Luizard, M.F.; Sigot, M.; Guidoin, R.; Domurado, D.; Blais, P.; Marois, M.; Gosselin, C.
Journal de Chirurgie 118(4): 269-274
1981
ISSN/ISBN: 0021-7697 PMID: 7228933 Document Number: 182066
Unlike synthetic arterial substitutes which are supplied as presterilized, dry-packaged products with potentially indefinite shelf lives, arterial prosthesis of modified biological origin must stored in less convenient liquid preservative media. These preservatives can affect the post-implantation performance and the biocompatibility of the devices. In order to identify the most suitable media, a modified "in vitro" cell culture test method was developed. It makes use of endothelial cells prepared from the aorta of chick embryo. In general, the prosthesis stored in liquid media had a disappointing performance from the point of view of neo-endothelium development and adhesion. Residual cytotoxicity was also noted for most of these currently used prosthesis storage and pre-surgical preparation protocols. Although devices stored in physiologic media showed adequate biocompatibility according to this cell culture test, it appears that neo endothelialization does not proceed concurrently with healing. Cellular development where present, adheres poorly to the substrate and cannot resist the shear forces of normal blood flow. Pre-implantation treatment of such prosthesis with cellular growth-promoting substances may enhance cell-prosthesis adhesion.