Histochemical analysis of urinary stone: part I--specificity and sensitivity of microchemical staining reactions to identify chemical constituents
Kabra, V.; Kabra, S.G.; Chaturvedi, R.P.; Bhargava, A.P.; Banerji, P.; Bhargava, A.; Rathore, M.S.
Indian Journal of Experimental Biology 19(11): 1050-1053
1981
ISSN/ISBN: 0019-5189 PMID: 7338361 Document Number: 181409
Available histochemical methods to stain Ca, phosphate, Mg, uric acid and oxalate were screened for their specificity to stain slide models of pure chemicals representing the known crystalline constituents of procedure specifically stains calcium oxalate brilliant red; Schultz procedure stains urate purple; Von Kossa procedure stains calcium phosphate (apatite) dark brown-black; and titan yellow dye method stains magnesium salts (chiefly struvite) orange to light red. Each staining procedure possesses sensitivity to stain the target chemical in 3% concentration and the proportion of the crystals stained in a slide in commensurate with the proportion in which they are present in the slide model. The histochemical procedure provides results comparable to X-ray diffraction analysis of stone for utilization in the management of cases of urinary lithiasis.