Allograft tolerance in pigs after fractionated lymphoid irradiation. II. Kidney graft after conventional total lymphoid irradiation and bone marrow cell grafting
Fradelizi, D.; Mahouy, G.; de Riberolles, C.; Lecompte, Y.; Alhomme, P.; Douard, M.C.; Chotin, G.; Martelli, H.; Daburon, F.; Vaiman, M.
Transplantation 31(5): 365-368
1981
ISSN/ISBN: 0041-1337 PMID: 7015623 Document Number: 180510
Experiments with pigs were performed to establish bone marrow chimerism and kidney graft tolerance between SLA 4 times a wk .times. 13 times (TLI) (2 animals) and B = 350 cGy 3 times a week .times. 8 times (TLI) (4 animals). Bone marrow cells were injected 24 h after the last irradiation. One day later, bilateral nephrectomy and the graft of 1 kidney from the bone marrow cell donor were performed simultaneously. No permanent chimerism was ever obtained except for 1 animal in protocol A which received marrow cells contaminated with blood lymphocytes and which developed an acute lethal graft-vs.-host reaction. A modification of protocol B, where the last 2 irradiations were administered on the whole body, was tested on 3 animals. It was equally unsuccessful in the establishment of chimerism. Despite the nondetectable chimerism, a partial tolerance to a semi-incompatible kidney was obtained in 2 cases with protocols A and B (survival 6 mo. and 7 mo.). An unexpectedly high incidence of accelerated kidney graft rejection was observed in several cases (acute renal failure as early as days 4, 5 and 6 after grafting). Success or acute failure of the graft could not be explained by the degree of histocompatibility or by the intensity of the immunosuppression, which were roughly equal in all pairs tested. Evidently, application of the TLI protocol to humans is not yet practicable and further experimental work is needed.