Retinol receptors in rat retina: various molecular forms
Pokrovskiĭ, B.V.; Shabanova, M.E.; Mitsner, B.I.
Voprosy Meditsinskoi Khimii 27(3): 392-397
1981
ISSN/ISBN: 0042-8809 PMID: 7197081 Document Number: 180402
Analysis of vitamin A-binding components in a soluble fraction of rat retina was carried out using gel filtration and gradient ultracentrifugation. Use of a wide range of concentrations of labelled retinol (6.10-8 = 2. 10-5 M) enabled to detect both high and low molecular forms of the binding components of retina. High molecular components (5S, 7S) were mainly found at physiological concentrations of retinol and at the higher (10-6=10-5 M) concentrations. Low molecular mass receptor (2S) prevailed under conditions of loading of the soluble fraction with subphysiological (nmol) concentrations of retinol.