The analysis of various protein in cerebrospinal fluid in optic nerve diseases
Nakajima, T.
Nippon Ganka Gakkai Zasshi 85(8): 941-951
1981
ISSN/ISBN: 0029-0203 PMID: 6976076 Document Number: 179591
The analysis of various proteins in CSF is important for a diagnosis of CNS- and optic nerve diseases. The CSF and serum proteins in patients with ophthalmic diseases were examined. The diagnosis of the patients were as follows; acute optic neuritis (16 cases), chronic optic neuritis (22 cases), Leber's hereditary optic atrophy (6 cases), rhinogenous optic neuritis (4 cases), uveitis (7 cases), brain tumor (8 cases), meningitis (4 cases), Fisher's syndrome (2 cases) and other diseases (4 cases). Prealbumin, albumin (Alb), .alpha.1-antitrypsin (.alpha.1AT), transferrin and IgG in the CSF and serum were analyzed by single radial immunodiffusion and .alpha.1-acid glycoprotein (.alpha.1AGP), and .alpha.2-macroglobulin were analyzed by electro-immunodiffusion. Since quantitative analysis for the .alpha.1AGP was most suitable by the CSF/serum .alpha.1AGP ratio and for other proteins concentration of those proteins in CSF were clarified previously, these methods were adopted. Quantitative change of various protein were observed in chronic optic neurtitis (11 cases) and Leber's hereditary optic atrophy in which the the surgical lysis of the chiasmal thikened arachnoid membrane was preformed. Two wk after the surgery .alpha.1AGP ratio and .alpha.1AT concentration showed significantly higher values compared to those before the surgery (P < 0.05 and P < 0.05). Relation .alpha.1AGP ratio as % of CSF Alb also showed significantly higher value than preoperative values (P < 0.05) and relation .alpha.1AT as % of CSF Alb of 2 and 3 wk after operation showed significantly higher value (P < 0.01 and P < 0.10). The .alpha.1AGP ratio and concentration of .alpha.1AT in CSF could be an index for slight disturbance of blood-CSF barrier. Cases of acute optic neuritis (16) were classified into 2 groups that showed both .alpha.1AT concentration and relative CSF IgG as % CSF Alb to be significantly higher than the control group. Blood-CSF barrier was apparently slightly damaged in the 1st group, and IgG synthesis within the CNS occured in 2nd group. In the rhinogenous optic neuritis, the CSF proteins showed no characteristic changes, and the sellar meningioma almost all proteins showed higher values than normal.