Comparison of granulation tissue formed beneath epithelial transplants with high and low bone-inducing potential
Moskalewski, S.; Ksiazek, T.; Malejczyk, J.
Folia Biologica 29(3-4): 177-184
1981
ISSN/ISBN: 0015-5497 PMID: 7343326 Document Number: 179360
Autogeneic transplants of urinary bladder and seminal vesicle wall were made in guinea pigs. Transitional epithelium induced bone in all 10 transplants lasting for 28 days, while epithelium of seminal vesicles yielded bone in 1 of 12 transplants of similar duration, displaying weak osteo-inductive capacity. Transplanted epithelia formed cysts with underlying granulation tissue. This tissue in the vicinity of both types of transplants contained well-developed collagen fibers and had a similar structural organization. The only regularly occurring difference was high alkaline phosphatase activity in fibroblasts accumulated near the growing transitional epithelium. Cells with such activity were already present in 5-day-old tranplants, while bone formation occurred on the 12th day. Judging by the localization of bone in relation to the bottom of the cyst, ossification occurred in areas of granulation tissue with high alkaline phosphatase activity. Alkaline phosphatase in granulation tissue may be induced by a factor released from the transitional epithelium. This factor could be an organic phosphate since such compounds are known to induce alkaline phosphatase in other systems.