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Alkylation of calf thymus DNA with reductively activated mitomycin C

Hashimoto, Y.; Shudo, K.; Okamoto, T.

Nucleic Acids Symposium Series 10: 217-220

1981

ISSN/ISBN: 0261-3166
PMID: 6796944
Document Number: 178321
Mitomycin C (MMC) was catalytically reduced in the presence of a nucleotide or calf thymus DNA. Reaction with 5'-guanylic acid (5'-GMP) gave 1,2-cis-2, 7-diamino-1-(5'-guanylyl) mitosene. Reaction with calf thymus DNA gave modified DNA, which on enzymatic hydrolysis gave two alkylated 5'-deoxyguanylic acid (MG-1 and MG-2) and an alkylated 5'-deoxyadenylic acid (MA). This is the first example of isolation of nucleotides from DNA modified by MMC.

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