Immunosorbent based on porous cellulose sphere
Gurvich, A.E.; Lekhttsind, E.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 92(12): 752-754
1981
ISSN/ISBN: 0365-9615 PMID: 7326430 Document Number: 177276
The synthesis of an immunosorbent in the form of porous beads is described. The beads were prepared by emulsification of cuprammonium glucose solution in organic solvents, followed by precipitation of beads with a mixture of acetone and sulfuric acid. The product was oxidized with NaIO4 and conjugated with protein antigen or antibodies to obtain an immunosorbent fit for column chromatography. The capacity of the immunosorbent thus obtained was found to be 300-1000 mg of antibodies or 50-70 mg of antigen per 1 g of the sorbent.