Serologic and biochemical identification of minor histocompatibility (H-4) antigens
Long, P.M.; Lafuse, W.P.; David, C.S.
Journal of Immunology 127(3): 825-828
1981
ISSN/ISBN: 0022-1767 PMID: 7264303 Document Number: 177040
Mouse alloantiserum capable of detecting minor histocompatibility antigens has been produced. Cytotoxic antibody to products of the H-4 locus was produced by reciprocal immunization of minor H loci congenic strains B10 (H-4a) and B10.129(21M) (H-4b), with mitogen-stimulated lymphocytes and epidermal cells. Absorption studies suggested antibodies to more than 1 specificity. The antibodies cross-react with products of other minor H loci. Alloantiserum to the H-4 gene product precipitates a glycoprotein with a m.w. of approximately 45,000 from radiolabeled Con A-stimulated lymph node cells.