Thermal Steam Aerosolization Protects Against Lipopolysaccharide-Induced Sepsis in Rats
Matsathit, U.; Mekseepralard, C.; Wongsawatkul, O.; Ratanachamnong, P.; Upapan, P.; Phivthong-Ngam, L.
Journal of the Medical Association of Thailand 99(Suppl 8): S206-S215
2016
ISSN/ISBN: 0125-2208 PMID: 29906046 Document Number: 17577
Sepsis is a severe systemic inflammatory state in response to infection. The induction of heat shock protein 70 The present study tested the hypothesis that thermal steam aerosolization could protect against lipopolysaccharideinduced sepsis in rats. Male Spraque-Dawley rats were subjected to steam aerosolization at 40°C for 1 hour before Thermal steam aerosolization treatment prevented the fall in systolic, diastolic, mean arterial blood pressures induced by LPS and restored the vascular response to adrenaline. LPS significantly increased plasma nitrate/nitrite concentration, serum IL-1 beta and IL-6 levels, and NF-kappa B activity in rat lung lysate which were reduced by thermal steam aerosolization. Thermal steam aerosolization induced both HSP70 and HSF-1 mRNA expression. The present study suggests that thermal steam aerosolization can delay the stage of shock in LPS-induced septic rats. It shows a beneficial therapeutic effect and may be applied to the clinical approach for septic shock patient.