Cellular killing of microfilariae of Onchocerca volvulus: eosinophil and neutrophil-mediated immune serum-dependent destruction
Greene, B.M.; Taylor, H.R.; Aikawa, M.
Journal of Immunology 127(4): 1611-1618
1981
ISSN/ISBN: 0022-1767 PMID: 7276574 Document Number: 175112
A system that demonstrates in vitro cell-mediated killing of microfilariae of O. volvulus was developed, using cryopreserved -deficient, and heat-inactivated guinea pig serum. Using purified eosinophils, the absolute increase in the percentages killed were GPS 40 .+-. 12, C4D-GPS 26 .+-. 4; with neutrophils GPS 23 .+-. 4, C4D-GPS 17 .+-. 2. The biological relevance of Mf obtained from cryopreserved nodules was confirmed in experiments using fresh skin-derived Mf and cells from infected individuals in West Africa. These experiments gave similar results except that cell-mediated killing was consistently increased by the addition of fresh serum alone, without immune serum.