Morphofunctional characteristics of mouse liver mitochondria (morphologic and biochemical study)
Avtandilov, G.G.; Sirotkin, V.V.; Kozyreva, E.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 92(8): 96-97
1981
ISSN/ISBN: 0365-9615 PMID: 7295971 Document Number: 173069
A study was made of a correlation between the size of intact mitochondria and biochemical parameters of their activity. A suspension prepared from mitochondria isolated from the linear mouse liver at varying times after gamma-irradiation was on object of investigation. Electron microscopy was performed along with a polarographic examination of mitochondrial respiration and oxidative phosphorylation. It was shown that the negative contrast examination of mitochondria followed by quantitative analysis of their appearances on the electron microscopic photographs enables making an accurate enough and objective judgement of mitochondrial functional activity.