Modification of platelet function by radical species produced during irradiation of oxygenated water
Krinsky, N.I.; Sladdin, D.G.; Levine, P.H.; Taub, I.A.; Simic, M.G.
Thrombosis and Haemostasis 45(2): 116-120
1981
ISSN/ISBN: 0340-6245 PMID: 6266067 Document Number: 171326
Human gel-filtered platelets in neutral, O2-containing solutions irradiated to a dose of 10 krad with .gamma.-rays display a significant inhibition of ADP-induced aggregation. Though the superoxide anion radical (O2-.cntdot.) and H2O2 are generated in water irradiated under these conditions, only catalase, but not superoxide dismutase (SOD), conveyed protection against this inhibition of platelet function. When the platelets are in the presence of 10-3 M sodium formate, which converts the majority of the radical species formed to O2-.cntdot., inhibition of aggregation was observed. Under these circumstances, the addition of catalase significantly decreased this inhibition, whereas the addition of SOD was without effect. To separate the platelet-inhibition effects of the radical species generated in the medium from a direct effect of irradiation on platelet components, relative stable quantities of O2-.cntdot. were produced in alkaline solutions and reacted with gel-filtered platelets. An inhibition of ADP-induced aggregation was observed. This inhibition was not significantly altered by the addition of SOD, but was abolished by the addition of catalase, either with or without SOD. O2-.cntdot. apparently is without direct effect on platelet function, but serves as the precursor of H2O2, which can inhibit platelet reactions. Phagocytosing polymorphonuclear leukocytes can produce a sufficient quantity of H2O2 to inhibit functioning of nearby platelets.