Xanthones Isolated from the Pericarp of Mangosteen Inhibit Neurotransmitter Receptors Expressed in Xenopus Oocytes
Leewanich, P.; Suksamram, S.
Journal of the Medical Association of Thailand 98(Suppl 10): S118-S123
2015
ISSN/ISBN: 0125-2208 PMID: 27276843 Document Number: 17029
This study aimed to evaluate inhibitory effects of 7 xanthones and 3 extracts obtained from the pericarp of mangosteen on serotonin (5-HT), N-methyl-D-aspartate (NMDA) and glycine receptors expressed in Xenopus oocytes. Xenopus oocytes were injected with RNA of either 5-HT NMDA or glycine receptor and inhibitory effects of the xanthones and extracts were investigated using the two-electrode voltage clamp technique. Xanthones from the pericarp of mangosteen affected 5-HT NMDA and glycine receptor functions with different degree of inhibition. Alpha-mangostin, garcinone-D and 9-hydroxycalaba xanthone inhibited 5-H T-induced currents by more than 80%. Gamma-mangostin and garcinone-E did by more than 50%, but not more than 80%. Garcinone-C and garcinone- D inhibited glutamate-induced currents by more than 80%. The alcohol extract did by more than 50%, but not more than 80%. Alpha-mangostin, garcinone-C, garcinone-D, non-tannin extract and the alcohol extract inhibited glycine-induced currents between 50-70%, neither compounds inhibited the currents up to 80%. These results suggest that each xanthone derivatives has different selectivity to different types of neurotransmitter receptors.