Partial purification of a common antigen in bovine lymphoma and its use in a lymphocyte blastogenesis assay
Jacobs, R.M.; Valli, V.E.; Wilkie, B.N.; Hollinshead, A.C.
Cancer Research 41(8): 3000-3004
1981
ISSN/ISBN: 0008-5472 PMID: 6265072 Document Number: 169697
An antigen was isolated from tumor cells derived from cases of bovine lymphoma which caused the in vitro blastogenesis of lymphocytes from 9 of 13 (69%) adult cattle with lymphoma. Lymphocytes derived from 5 of 122 (4%) normal adult cattle also underwent blastogenesis while lymphocytes from 3 cases of the sporadic form of lymphoma did not respond. Blastogenesis of lymphocytes from normal cattle may have resulted from alloantigen activity in the antigen; normal lymph node antigen did not stimulate lymphocytes from normal adult cows or cows with lymphoma. Antigen derived from tumor was localized to the cell membrane and cytoplasm of tumor cells by indirect immunofluorescent staining using an antiserum prepared in rabbits. Specific fluorescence was reduced by the absorption of this antiserum with a crude tumor extract. This antiserum did not cross-react with normal lymph node antigen or.