Cyclic AMP concentrations in human peripheral blood lymphocytes. Changes in association with cell purification
Goffstein, B.J.; Gordon, L.K.; Wedner, H.J.; Atkinson, J.P.
Journal of Laboratory and Clinical Medicine 96(6): 1002-1041
1980
ISSN/ISBN: 0022-2143 PMID: 6253577 Document Number: 167505
In studies over a several year period of cAMP in human peripheral blood lymphocytes, a wide range of concentrations has been from low values (1 to 5 pmole/10(7) cells) to high values (80 to 160). Evaluation of this variation indicated that between cell separation and completion of the experiment, there eas a progressive decrease in lymphocyte cAMP as determined by RIA or immunocytochemistry. After a 2 hr incubation at 37 degrees C, a new steady state was reached at 5% to 30% of the initial concentration. This phenomenon was unique to lymphocytes and not preventable by manipulation of the incubation conditions. The cause of the cAMP reduction was shown to be decreasing AC activity. These data suggested the possibility that AC was activated during cell isolation. However, the phenomenon was present in cells not exposed to dextran or Ficoll-Hypaque, and simulation of conditions employed during purification did not result in increased cAMP. Consequently, high-lymphocyte cAMP concentrations may be the normal levels in vivo.