Peanut agglutinin. V. Thymocyte subpopulations in the mouse studied with peanut agglutinin and Ly-6.2 antiserum
London, J.; Horton, M.A.
Journal of Immunology 124(4): 1803-1807
1980
ISSN/ISBN: 0022-1767 PMID: 6988508 Document Number: 167376
Thymocyte heterogeneity was studied by the use of 2 cell surface markers.sbd.the Ly-6.2 alloantigen and peanut agglutinin (PNA) receptor. The previously described distribution of Ly-6 on corticoresistant and PNA binding to corticosensitive thymocytes was confirmed. Double immunofluorescence labeling experiments in combination with techniques of physical enrichment for mature thymocytes resulted in the identification of a novel subset of T [thymus-derived] cells carrying the Ly-6 antigen and PNA receptors. Purification by fluorescence cell sorting of corticosteroid-treated thymocytes, labeled with monoclonal anti-Ly-2.2 antiserum, for phemotypically mature Ly-1+23- lymphocytes caused concomitant enrichment in the proportion of Ly-6-positive cells, which lack the PNA receptor. The implications of these findings for the maturational pathways within the thymus were discussed.