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Purification and properties of hamster liver ligandins, glutathione S-transferases

Smith, G.J.; Ohl, V.S.; Litwack, G.

Cancer Research 40(6): 1787-1790

1980

ISSN/ISBN: 0008-5472
PMID: 7371010
Document Number: 166142
Glutathione S-transferases have been purified to homogeneity from Chinese hamster liver. Three enzyme forms were separated and designated Forms I, II, and III in order of their elution from carboxymethylcellulose columns. The forms exhibit close physical similarities to glutathione S-transferases B (ligandin) of rat liver and epsilon of humam liver. However, enzyme kinetic analysis indicates that the hamster enzymes exhibit similar Km values but higher Vmax values towards common substrates compared with the rat and human forms. These differences, which explain the increased enzymic activities of hamster glutathione S-transferases in vivo and in vitro, appear to be related to slight differences in the peptide composition of hamster liver glutathione S-transferases compared to the rat and human enzymes.

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Dierickx, P.J.; Almar, M.M.; De Jonckheere, J.F. 1990: Glutathione transferase activity in some flagellates and amoebae, and purification of the soluble glutathione transferases from Acanthamoeba Biochemistry International 22(4): 593-600
Hall, A.G.; Matheson, E.; Hickson, I.D.; Foster, S.A.; Hogarth, L. 1994: Purification of an alpha class glutathione S-transferase from melphalan-resistant Chinese hamster ovary cells and demonstration of its ability to catalyze melphalan-glutathione adduct formation Cancer Research 54(13): 3369-3372
Ublacker, G.A.; Johnson, J.A.; Siegel, F.L.; Mulcahy, R.T. 1991: Influence of glutathione S-transferases on cellular glutathione determination by flow cytometry using monochlorobimane Cancer Research 51(7): 1783-1788
Bushma, M.I.; Legon'kova, L.F.; Zavodnik, L.B.; Zverinskiĭ, I.V.; Lukienko, P.I. 1994: The protective action of a combination of essentiale with diethylnicotinamid on rat liver mono-oxygenases and glucuronyl- and glutathione transferases in a trichloromethane lesion Eksperimental'naia i Klinicheskaia Farmakologiia 57(4): 62-63
Scott, T.R.; Kirsch, R.E. 1991: Glutathione S-transferases YcYfetus and YcYc--kinetic and inhibitor studies relating to their glutathione peroxidase activities South African Medical Journal 79(6): 298-301
Cmarik, J.L.; Inskeep, P.B.; Meredith, M.J.; Meyer, D.J.; Ketterer, B.; Guengerich, F.P. 1990: Selectivity of rat and human glutathione S-transferases in activation of ethylene dibromide by glutathione conjugation and DNA binding and induction of unscheduled DNA synthesis in human hepatocytes Cancer Research 50(9): 2747-2752
Robertson, I.G.; Guthenberg, C.; Mannervik, B.; Jernström, B. 1986: Differences in stereoselectivity and catalytic efficiency of three human glutathione transferases in the conjugation of glutathione with 7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene Cancer Research 46(5): 2220-2224
Takahashi, Y.; Niitsu, Y. 1994: Glutathione S transferases-pi Gan to Kagaku Ryoho. Cancer and ChemoTherapy 21(7): 945-951
Mikstacka, R. 1988: The role of glutathione transferases in inactivation of carcinogenic compounds Postepy Biochemii 34(1-2): 47-57
Anttila, S.; Hirvonen, A.; Vainio, H.; Husgafvel-Pursiainen, K.; Hayes, J.D.; Ketterer, B. 1993: Immunohistochemical localization of glutathione S-transferases in human lung Cancer Research 53(23): 5643-5648
Baars, A.J.; Breimer, D.D. 1980: The glutathione S-transferases: their role in detoxification and toxification of xenobiotics Annales de Biologie Clinique 38(1): 49-56
Cook, J.A.; Iype, S.N.; Mitchell, J.B. 1991: Differential specificity of monochlorobimane for isozymes of human and rodent glutathione S-transferases Cancer Research 51(6): 1606-1612
Board, P.G.; Chelvanayagam, G.; Jermiin, L.S.; Tetlow, N.; Tzeng, H.F.; Anders, M.W.; Blackburn, A.C. 2001: Identification of novel glutathione transferases and polymorphic variants by expressed sequence tag database analysis Drug Metabolism and Disposition: the Biological Fate of Chemicals 29(4 Pt 2): 544-547
Salagovic, J.; Kalina, I.; Stubna, J.; Habalová, V.; Hrivnák, M.; Valanský, L.; Kohút, A.; Biros, E. 1998: Genetic polymorphism of glutathione S-transferases M1 and T1 as a risk factor in lung and bladder cancers Neoplasma 45(5): 312-317
Tarasova, G.I.; Lukomskaia, I.S. 1970: Purification and properties of rabbit liver trehalase Biokhimiia 35(1): 102-108
Marie, J.P.; Simonin, G.; Legrand, O.; Delmer, A.; Faussat, A.M.; Lewis, A.D.; Sikic, B.I.; Zittoun, R. 1995: Glutathione-S-transferases pi, alpha, mu and mdr1 mRNA expression in normal lymphocytes and chronic lymphocytic leukemia Leukemia 9(10): 1742-1747
Chatterjee, U.; Kumar, A.; Sanwal, G.G. 1989: Purification and properties of goat liver catalase: two pH optima Indian Journal of Biochemistry and Biophysics 26(3): 140-147
Hayes, J.D.; McLeod, R.; Ellis, E.M.; Pulford, D.J.; Ireland, L.S.; McLellan, L.I.; Judah, D.J.; Manson, M.M.; Neal, G.E. 1996: Regulation of glutathione S-transferases and aldehyde reductase by chemoprotectors: studies of mechanisms responsible for inducible resistance to aflatoxin B1 Iarc Scientific Publications 139: 175-187
Peters, W.H.; Roelofs, H.M. 1992: Biochemical characterization of resistance to mitoxantrone and adriamycin in Caco-2 human colon adenocarcinoma cells: a possible role for glutathione S-transferases Cancer Research 52(7): 1886-1890
Dostal, L.A.; Guthenberg, C.; Mannervik, B.; Bend, J.R. 1988: Stereoselectivity and regioselectivity of purified human glutathione transferases pi, alpha-epsilon, and mu with alkene and polycyclic arene oxide substrates Drug Metabolism and Disposition: the Biological Fate of Chemicals 16(3): 420-424