Effect of gonadotrophin inhibiting material isolated from human urine on action of prolactin at rat Leydig cell
Bagli, N.P.; Rajendran, K.G.; Shah, P.N.
Indian Journal of Experimental Biology 18(5): 456-458
1980
ISSN/ISBN: 0019-5189 PMID: 7429558 Document Number: 165369
To study the effect of gonadotropin inhibiting material (GIM) on the binding of prolactin [P] to Leydig cell receptors isolated Leydig cells were incubated with 125I-P. Presence of GIM in the incubation mixture did not inhibit the binding of 125I-P to Leydig cells whereas unlabeled P significantly reduced the binding. In another experiment, testicular cells were incubated with fluorescein isothiocyanate-tagged GIM. GIM binding to Leydig cells was seen by the presence of fluorescence on these cells. This binding could be inhibited by untagged GIM but not by P. The results apparently suggest the presence of separate receptors for GIM and P on the Leydig cells and indicate that termination of pregnancy by GIM is not due to any interference with P binding to its receptors.