Myocardial changes in experimental coronary perfusion under conditions of normothermia and hypothermia. I. Biochemical studies
Furman, M.; Andrzejewska, A.; Kurasz, S.; Zimnoch, L.; Cylwik, B.
Kardiologia Polska 23(3): 205-212
1980
ISSN/ISBN: 0022-9032 PMID: 7373970 Document Number: 164073
Isolated hearts obtained from dogs were perfused in extracorporeal circulation in 3 groups: under mormothermia, at 20.degree. C and at 4.degree. C. After 3 h of perfusion phosphocreatine (CP), ATP, lactic acid and pH, H2O content and Na and K concentrations were determined in the myocardium. Perfusion under normothermia produced myocardial swelling with a rise in H2O content by 9.3% reduced CP to 34.8% and ATP to 68.9% of the control values, raised lactic acid concentration from 4.95 to 12.32 .mu.mol/g of myocardial tissue, pH lowering to 7.20, reduced concentration of K and raised concentration of Na. Following perfusion under hypothermia at 20.degree. C and 4.degree. C biochemical disturbances were much less and CP and ATP were unchanged. Perfusion associated with hypothermia provided a better protection of the myocardium during prolonged operations on the heart or during heart storage when the heart is taken for transplantation than normothermic perfusion.