Effect of experimental myocardial infarct on the rate of NADH and 3-hydroxybutyrate oxidation in heart mitochondria
Dzheia, P.P.; Toleikis, A.I.; Prashkiavichius, A.K.
Voprosy Meditsinskoi Khimii 26(6): 731-735
1980
ISSN/ISBN: 0042-8809 PMID: 7456403 Document Number: 162639
The rate of oxidation of NADH and 3-hydroxybutyrate was studied in heart mitochondria after short-term (60 min) occlusion of coronary artery and the subsequent reperfusion. Addition of NAD increased the rate of 3-hydroxybutyrate oxidation, lowered in mitochondria of impaired tissue, but no complete restoration occurred. Th 3-hydroxybutyrate dehydrogenase activity was decreased by 25%. The rate of NADH oxidation in ultrasonicated mitochondria from the infarction zone exceeded 2-fold the rate of 3-hydroxybutyrate oxidation. These data suggest that deficiency of NAD limited primarily the rate of 3-hydroxybutyrate oxidation in heart mitochondria under conditions of experimental myocardial infarction. The level of 3-hydroxybutyrate dehydrogenase activity appears to restrict the rate of 3-hydroxybutyrate oxidation after addition of NAD.