Effect of low molecular weight dextran sulphate on plasmin activity measured with S-2251
Matsuo, O.; Kawaguchi, T.; Kosugi, T.; Mihara, H.
Acta Physiologica Latino Americana 30(3): 193-198
1980
ISSN/ISBN: 0001-6764 PMID: 6180601 Document Number: 162292
The effect of low molecular weight (LMW) dextran sulfate (DS) (MW 3900) on plasminogen activation by urokinase was investigated. When human plasma containing urokinase (100 IU/ml) was diluted with DS solution and acidified to pH 4, marked splitting activity of the substrate D-Val-Leu-Lys-pNA [S-2251], which is a specific substrate for plasmin, appeared. The precipitate obtained after centrifugation of diluted and acidified plasma exhibited remarkable substrate splitting activity. Activation of plasminogen to plasmin by urokinase was enhanced by the LMW DS.