Genetic properties of phage lambda recombinant molecules. the effect of an inserted fragment on the hybrid yield and recombination with respect to the h- and vir-markers
Maliuta, S.S.; Danilenko, T.S.; Pererva, T.P.
Tsitologiia i Genetika 14(5): 73-76
1980
ISSN/ISBN: 0564-3783 PMID: 6449098 Document Number: 161839
The yield of mature phages in lambda gt-lambda Dm34, lambda gt-lambda DM225 and lambda gt-lambda Dm234 is 2, 8 and 8 times lower (respectively) as compared to the wild type. The output of hvir-recombinants in lambda gt-lambda Dm225 is more than an order lower as compared to the other phages. As distinct from two other hybrids, the decrease in lambda gt-lamba DM225 yield cannot be explained by red-genotype and a shortening of the phage genome as well as the decrease in the output hvir-recombinants cannot be attributed to a decrease in the yield of mature particles and to a shortening of the distance between markers. The data obtained show a contribution of Drosophila DNA to the observed effects.