Isolation of Chlamydia psittaci from bull ejaculate
Trávnicek, M.; Balascák, J.; Balatý, B.; Dravecký, T.
Veterinarni Medicina 25(11): 669-673
1980
ISSN/ISBN: 0375-8427 PMID: 6777949 Document Number: 161787
During the repeated serological examination (RVK) in five breeding bulls the positive levels of antibodies to Chlamydia psittaci in titre 1 : 128 were found. In the isolation experiments the pelleted ejaculates deposited in liquid nitrogen were used. The isolation of Chlamydia psittaci on yolk sacs of chicken embryos was positive in two breeding bulls. The isolated strains are labelled GN-33 and OK-107. The serological examination of blood samples was in all five breeding bulls negative on brucellosis (BAB), infectious bovine rhinotracheitis and coxiellosis and positive on PI-3. Bacteriological examination of spermatic fluid proved only sporadic contamination with moulds and saprophytic bacteria.