Practical staining method: T-OPA triple stain
Tajana, G.; Marotta, M.; De Palma, L.
Bollettino della Societa Italiana di Biologia Sperimentale 56(19): 2026-2029
1980
ISSN/ISBN: 0037-8771 PMID: 6161625 Document Number: 161723
The following procedure has proven to be successful as routine trichrome stain on paraffin embedded material: 1) Mayer's hemalum for 10 min, followed by running tap water wash; 2) staining in 1% Orange G in 1% acqueous PTA for 5 min and rinsing a few seconds in distilled water; 3) Aniline blue 1% acqueous for 5 min, followed by few seconds distilled water wash. Dehidratation in ethanol, or by blotting followed by t-buthanol or 1:3 terpineol-xylene, clearing and mounting, completed the procedure.