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Persistence of subpopulations of a strain of influenza virus in a culture of L cells

Sidorenko, E.V.; Maksimovich, N.A.; Taĭkova, N.V.; Grashchenko, T.B.; Vasina, A.G.

Mikrobiolohichnyi Zhurnal 42(3): 353-359

1980

ISSN/ISBN: 0201-8462
PMID: 7402111
Document Number: 161276
Using DEAE-Sephadex A-50 chromatography, the whole population of influenza A (Hong Kong) 1/68 virus was divided into subpopulations. Inoculation of mouse fibroblast L cells with them and subsequent passage resulted in the formation of persistent infection. During passage (152 days) differences were found between the sublines in localization of a specific antigen detected by the immunofluorescent method. The viruses isolated from the persistently infected cells differed in biologic properties.

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