Immunopharmacological controls of indirect side-effects in antibiotherapy
Rojo, J.M.; Barasoain, I.; Jorba, G.; Portolés, A.
Archivos de Farmacologia y Toxicologia 6(2): 107-118
1980
ISSN/ISBN: 0304-8616 PMID: 7469494 Document Number: 161074
Modifications affecting the host immune response are probably the less known among the secondary side effects induced by antibiotics. Different tests (4) are proposed to control the immunopharmacological behavior of several antibiotic molecules. These assays were: evaluation of the polyclonal activation response of lymphoid cells; control of variations in the synthesis of antibodies in the splenocytes; stimulation of the in vivo effects on a phagocytic system of peritoneal macrophages and control of the potential capacity to elicit antibiotic hypersensitive reactions. Mitomycin, rifamycin, tetracycline, chloramphenicol and streptomycin were the most active antibiotics in diminishing the 3H-thymidine uptake during lymphoblast transformation; among nucleic acid inhibitors, the most potent suppressor of antibody synthesis was rifamycin, but actinomycin affected the IgM-producing cells more strongly; in the presence of protein inhibitors the phagocyte response was most diminished by chloramphenicol, and the capacity of immunosensitization of the .beta.-lactam molecules may be modified in the presence of adjuvants or by the method of treatment.