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Short term cultivation of Babesia species

Timms, P.

Research in Veterinary Science 29(1): 102-104

1980

ISSN/ISBN: 0034-5288
PMID: 7455338
Document Number: 159679
A method based on the candle jar technique was used for the cultivation of B. bovis, B. bigemina and B. rodhiani in vitro. All 3 parasites multiplied within their host red blood cells and were well maintained for up to 96 h in culture. Regular changes of medium and subculture at 24 h were essential for optimal parasite survival. A 40% suspension of red blood cells gave better results than the 10-12% suggested by Jensen and Trager for the cultivation of Plasmodium falciparum.

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