Experimental pancreatitis in the rat. The role of phospholipase A in sodium taurocholate-induced acute haemorrhagic pancreatitis
Aho, H.J.; Nevalainen, T.J.; Lindberg, R.L.; Aho, A.J.
Scandinavian Journal of Gastroenterology 15(8): 1027-1031
1980
ISSN/ISBN: 0036-5521 PMID: 6165066 Document Number: 159638
Acute hemorrhagic pancreatitis was induced in rats by injecting 0.2 ml of 5% (92.9 mmol/l) aqueous solution of sodium taurocholate into the common biliopancreatic duct. Lysolecithin was separated from the pancreatic homogenate by TLC and quantified by P determination. The lysolecithin content increased rapidly, remained elevated for 12 h and returned to the control level 24 h after the injection. Treatment with a trypsin inhibitor, aprotinin (Trasylol), given i.p. and i.v. during 2 h postoperatively (800,000 units/kg of body weight) had no beneficial effects compared with physiological saline treatment. When the animals were treated similarly with a phospholipase A inhibitor, procaine hydrochloride (40 mg/kg of body weight), 45% of them survived 72 h (P < 0.01). Phospholipase A, which converts lecithin into lysolecithin, evidently plays a significant role in the pathogenesis of acute pancreatitis.