Inhibitory effect on the formation of chlamydial inclusions in McCoy cells by seminal fluid and some of its components
Mårdh, P.A.; Colleen, S.; Sylwan, J.
Investigative Urology 17(6): 510-513
1980
ISSN/ISBN: 0021-0005 PMID: 6246025 Document Number: 159007
The effect of semen and some of its components upon chlamydial inclusion (CI) count and cytopathogenic effect in cycloheximide-treated McCoy cells. Semen and spermine caused a dose-related decrease in the number of CI, using a standardized infectious dose of Chlamydia trachomatis, immunotype F. Lysozyme in certain concentrations stimulated CI formation, whereas the cations Cu++ and Zn++ produced a cytopathogenic effect and a dose-related reduction of the CI count. EDTA neutralized the effects of the cations and its addition to seminal fluid experimentally infected with C. trachomatis resulted in a greater number of CI than found in control cultures containing only semen.