Effect of anticancer agents on the susceptibility of tumor cells to immune cytolysis
Nakao, S.; Fujii, G.
Japanese Journal of Experimental Medicine 50(6): 435-443
1980
ISSN/ISBN: 0021-5031 PMID: 7277785 Document Number: 158908
Effect of anticancer agents on the immune cytolysis of tumor cells was investigated. Cell of ascites hepatoma line of C3H/He strain of mouse, MH134, were treated with anticancer agents and used for target cells in immune cytolysis experiments. In cytotoxicity tests, MH134 cells pretreated with mitomycin C or Adriamycin were lysed by xenogeneic or allogeneic antisera and complement at significantly higher rates than non-treated MH134 cells. To test cytostatic effect of antisera, target MH134 cells were pretreated with much lower concentration of anticancer agents than that in the cytotoxicity tests, the anticancer-agent-treated tumor cells were damaged with complement and the xenogeneic and allogeneic antisera at higher rates than the non-treated tumor cells. Allogeneic spleen cells from DDD mice sensitized with MH134 tumor damaged anticancer-agent-treated MH134 cells at higher rates than non-treated MH134 cells. These results suggested an enhancing effect of anticancer agents on immune cytolysis of tumor cells in hosts who could actively and/or passively immunologically respond against tumor.