Surface glycoproteins of human sarcoma- and fibroblastic cells
Lubitz, W.; Grófová, M.; Pontén, J.; Popovic, M.
Neoplasma 27(6): 633-639
1980
ISSN/ISBN: 0028-2685 PMID: 6942238 Document Number: 158783
A comparison was made of the cell surface glycoproteins of four human cell lines, namely a giant tumor of bone cell line, an osteosarcoma line, a fibrosarcoma line and a human fibroblast line. The cells were labeled by lactoperoxidase catalyzed iodination and the glycoproteins extracted by 0.5% Triton X-100 were bound to lentil-lectin and subsequently analyzed by SDS gel electrophoresis. While the cell lines examined shared a series of common glycoproteins, it was found that the giant cell tumor line and the fibrosarcoma lines exhibited a higher degree of homology than the other cell lines.