Liposome immunosensor for theophylline
Haga, M.; Itagaki, H.; Sugawara, S.; Okano, T.
Biochemical and Biophysical Research Communications 95(1): 187-192
1980
ISSN/ISBN: 0006-291X PMID: 6998461 Document Number: 158749
A new simple, sensitive liposome immunosensor (LIS) was developed by combining the advantages of spin membrane immunoassay (SMIA) and enzyme immunosensor (EIS). The LIS system is composed of an O2 electrode and sensitized liposomes. It records liposome lysis induced by specific anti-theophylline antibodies and complement which is monitored by the release of entrapped enzymes instead of spin labels. A sensitive detection was performed due to the amplification of antigen-antibody reaction by liposome lysis and enzymatic reaction. The method offers a simple and sensitive quantitative detection of theophylline down to 4 .times. 10-9 M (0.7 ng/ml).