Methods for the measurement of multiple parameters of erythroid regulation within individual mice
Dunn, C.D.; Lange, R.D.
Laboratory Animal Science 30(6): 997-1002
1980
ISSN/ISBN: 0023-6764 PMID: 7464036 Document Number: 158249
Hematocrit, hemoglobin p50 (partial pressure of O2 at 50% saturation of Hb), percent reticulocytes, rate of erythropoiesis using 59Fe and blood volume were determined in individual mice using a single blood sample. Sufficient blood remained to provide a serum pool from no more than 5 mice for an erythropoietin assay. These multiple procedures did not interfere with one another, and the various measurements did not need to be made immediately. Freshly prepared 51Cr-labeled erythrocytes were required for blood volume determinations.