Fractionation of soluble molybdenum-binding proteins from liver, kidney, plasma and erythrocytes from sheep supplemented with molybdenum
Norheim, G.; Søli, N.E.; Frøslie, A.; Mjør-Grimsrud, M.
Acta Veterinaria Scandinavica 21(3): 428-437
1980
ISSN/ISBN: 0044-605X PMID: 7446324 Document Number: 158025
Ewes (5) were given a daily dose of 1.4 mg Mo/kg body weight as ammonium molybdate for 21-55 days. Plasma Mo [reported to counteract chronic Cu poisoning] concentrations increased following the dosage but no systematic changes were seen in plasma Cu concentrations. About 80% of Mo present in blood was found in plasma. Gel filtration showed that most of the Mo present in plasma and red cell hemolysates was found in a fraction with MW < 1500. Soluble proteins from liver and kidney cortex were separated into 1 high-MW fraction with MW > 200,000 and a non-protein fraction with MW < 1500. Significant correlations were found between the amounts of Mo present in the high-MW fraction and Mo concentrations in liver and kidney.