Isolation of a membranous fraction of toad gastric mucosa
García de Bohórquez, E.; Martínez, M.; Chacín, J.
Acta Physiologica Latino Americana 30(3): 153-158
1980
ISSN/ISBN: 0001-6764 PMID: 6287808 Document Number: 157380
In the present work the isolation and some characteristics of a membrane fraction from toad gastric mucosa are described. By combining differential centrifugation in a discontinuous gradient of sucrose it was possible to obtain a band at the interphase of 22% sucrose 37%, whose enzymatic and electron microscopic analysis indicated to be a fraction enriched of luminal plasma membranes from oxyntic cells, with very poor contamination by other subcellular organelles. This is a suitable membrane preparation that can be used to perform studied on the mechanisms of acid secretion in toads.