Estimation of antibodies to human cytomegalovirus by immunofluorescence and radioimmunoassay
Jankowski, M.; Gut, W.; Nawrocka, E.
Acta Microbiologica Polonica 29(3): 285-292
1980
ISSN/ISBN: 0137-1320 PMID: 19852114 Document Number: 156030
The 125J labeled IgG fraction against rabbit IgG of goat origin was employed for the detection of CMV IgG and IgM antibodies in the double indirect radioimmunoassay. The results were compared with those obtained in complement fixation, indirect immunofluorescence and anti-complement immunofluorescence tests. The application of labeled anti-fc antisera, instead of antisera against whole IgG in the tests for detection of specific CMV IgG antibody resulted in increased sensitivity of radioimmunoassay and reduction of non-specific cytoplasmatic reactions in preparations stained by indirect immunofluorescence. The absorption of sera with protein A rich staphylococci and aggregates to immunoglobulin eliminated unwanted secondary IgM staining caused by rheumatoid factors both in indirect immunofluorescence and radioimmunoassay tests for CMV antibodies.