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Activation of plasminogen with immobilized trypsin

Eretskaia, E.V.; Kudinov, S.A.

Ukrainskii Biokhimicheskii Zhurnal 51(4): 335-339

1979

ISSN/ISBN: 0201-8470
PMID: 38546
Document Number: 151316
Human plasminogen isolated from the placenta serum fraction by means of affinity chromatography was activated by trypsin being in covalent bond with sepharose. The activation is studied as dependent on pH, temperature and the proenzyme-activator ratio in the presence of 25% glycerol as a stabilizing agent and without it. Utilization of the immobilized trypsin as a plasminogen activator makes it possible to transform completely the proenzyme to plasmin varying the plasminogen-trypsin ratio and time of activation when it is conducted under optimal conditions: in the presence of 25% glycerol at pH 7.0-7.1 and the temperature of 30 degrees C.

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