Effect of procaine on the loss of erythrocyte phospholipids during blood preservation in ACD-AG-stabilizer
Olthoff, D.; Kunze, D.; Olthoff, G.
Folia Haematologica 106(1): 117-127
1979
ISSN/ISBN: 0323-4347 PMID: 88385 Document Number: 148733
The phospholipid changes of erythrocytes were investigated following the storage under blood bank conditions up to 42 days (ACD-AG-stabilisator) without and with addition of procaine (25 mM). The phospholipid loss of erythrocytes is described as two-step occurrence. The rapid decrease in the first three days is followed by a further loss up to the end of the storage period. The concomitant increase of phospholipid fractions of supernatant shows that the erythrocyte membranes lose their phospholipids mainly by a passive way. For the further loss of phospholipids the action of a phospholipase A is adopted. The addition of procaine hinders strongly the phospholipid loss of erythrocytes. The clinical importance of these procaine action is shortly discussed.