Enzyme histochemistry of the small intestine in inherited ichthyosis
Neto, J.M.; Mello de Oliveira, J.A.
Acta Dermato-Venereologica 59(2): 161-165
1979
ISSN/ISBN: 0001-5555 PMID: 84496 Document Number: 148593
Enzyme histochemistry of biopsies from the small intestine of 5 patients with different forms of inherited ichthyosis and from 2 normal volunteers was performed. Of the patients, 2 had ichthyosis vulgaris, 2 had non-bullous congenital ichthyosiform erythroderma (CIE) and 1 had X-linked ichthyosis. The following enzymatic activities were examined: glucose-6-phosphate dehydrogenase (G6P-D), 6-phosphogluconate dehydrogenase (6PG-D), NADPH2 tetrazolium reductase (NADPH2-TR), fructose bisphosphate aldolase (ALD-A), lactate dehydrogenase (LD), cisaconitase (CA), isocitrate dehydrogenase (ICD), succinate dehydrogenase (SD), malate dehydrogenase (MD), NADH2-tetrazolium reductase (NADH2-TR), ATP-A I, ATP-A II, ATP-A III and ATP-A IV (ATPase at pH 9.4, 8.5, 7.4 and 6.3, respectively); ribose-5-phosphatase (R5P-A), dihydroorotate dehydrogenase (DHO-D), glutamate dehydrogenase (GLD), monoamine oxidase (MAO), .alpha.-glycerophosphate dehydrogenase (.alpha.GP-D), .beta.-hydroxybutyrate dehydrogenase (.beta.HOB-D); alkaline phosphatase (.alpha.GP-AI); acid phosphatase (.alpha.GP-AII) and naphtholacetate esterase (N.EST-A). No significant variations in the different enzymatic activities were found for the ichthyosis vulgaris and non-bullous CIE cases. More pronounced variations were found in X-linked ichthyosis, with a decrease in CA, ICD, R5P-A, .beta.HOB-D, GLD, .alpha.GP-A II and N.EST-A activity. SD activity was previously reported to be reduced in ichthyosis vulgaris and bullous CIE, but for these patients SD activity was equal to or higher than that of controls.