Front-face fluorometry of liquid samples
Eisinger, J.; Flores, J.
Analytical Biochemistry 94(1): 15-21
1979
ISSN/ISBN: 0003-2697 PMID: 464277 Document Number: 147767
Quantitative analytical spectrofluorometry is usually performed with solutions which are optically dilute at the excitation wavelength and employs square cuvettes for observation at right angles to the excitation beam. In many biochemical applications, particularly when studying flurophores in blood, front-face fluorometry of optically dense samples offers certain advantages which include fluoresence intensities which are an order of magnitude larger. The features which characterize quantitative flurometry employing these 2 geometries are compared and a design for a front-face cell and cell holder, suitable for use in any standard spectrofluorometer, was presented.