Immunotherapy of a murine ovarian carcinoma with Corynebacterium parvum and specific heteroantiserum. I. Activation of peritoneal cells to mediate antibody-dependent cytotoxicity
Bast, R.C.; Knapp, R.C.; Mitchell, A.K.; Thurston, J.G.; Tucker, R.W.; Schlossman, S.F.
Journal of Immunology 123(5): 1945-1951
1979
ISSN/ISBN: 0022-1767 PMID: 489967 Document Number: 143289
Immunotherapy with a combination of C. parvum [Propionibacterium acnes] and specific heteroantiserum is significantly more effective than treatment with either single agent in prolonging the survival of mice that received an i.p. injection of syngeneic murine ovarian carcinoma (MOT) cells. In vitro, a combination of C. parvum-activated peritoneal cells and specific heteroantiserum was significantly more effective than either single component is destroying 51Cr-labeled MOT cells in the absence of complement. Activation of peritoneal cells to produce lysis of tumor in the presence of specific antiserum peaked 3 to 7 days after a single injection of C. parvum and declined to baseline over 3 to 4 wk. With repeated i.p. injections of C. parvum at appropriate intervals, activation of peritoneal cells could be prolonged and augmented. Among the routes tested, only i.p. administration of C. parvum was effective, although activation of peritoneal cells for cooperation with heteroantiserum was observed over a broad range of i.p. dosage (0.45-4.20 mg). Administration of C. parvum by appropriate doses, routes and schedules can attract and activate a population of peritoneal effectors that mediate antibody-dependent cytotoxicity more effectively than resident peritoneal cells.