New fluorescence microscopic technic for the identification of Trichomonas vaginalis
Grossgebauer, K.; Kegel, M.
Der Hautarzt; Zeitschrift für Dermatologie Venerologie und verwandte Gebiete 30(8): 434-436
1979
ISSN/ISBN: 0017-8470 PMID: 315935 Document Number: 142480
A new fluorescent dye for the identification of trichomonas vaginalis is described. Specimens under investigation were mixed with 4',6-diamidino-2-phenylindol (Dapi) and examined using white light and UV-light. The combined use of the two techniques allows to recognize the mobile non fluorescing and the dead intensive yellow fluorescing trichomonads. Devitalized epithelial cells, bacteria and leucocytes in contrast to living trichomonas are stained yellow in a few minutes. This is of diagnostic help.