Extraction, cleanup, and quantitative determination of aflatoxins B1 ANd M1 in beef liver
Trucksess, M.W.; Stoloff, L.
Journal - Association of Official Analytical Chemists 62(5): 1080-1082
1979
ISSN/ISBN: 0004-5756 PMID: 528452 Document Number: 141645
A method for the determination of aflatoxin B1 in eggs was applicable for aflatoxin B1 in liver, but ineffective for aflatoxin M1 in liver because of poor recovery of added aflatoxin and interferences in thin layer chromatography. The method was modified by the addition of citric acid to the extracting solvent and ammonium sulfate to the extract solution for removing protein. The elution system for silica gel column cleanup was also changed by substituting methanol for acetone, and adding a step for confirmation of aflatoxin M1 identity. The method has been used successfully for survey and research on aflatoxin residues in animal tissues.