A simple technique for nonsurgical embryo transfer in mice
Moler, T.L.; Donahue, S.E.; Anderson, G.B.
Laboratory Animal Science 29(3): 353-356
1979
ISSN/ISBN: 0023-6764 PMID: 502460 Document Number: 141150
A simple method was developed for nonsurgical transfer of mouse embryos which enabled transfer to both uterine horns. Embryos were picked up in a modified capillary tube and transferred through the cervix into each uterine horn of an unanesthetized mouse. A glass speculum was used to facilitate location of the cervix. The technique was found to be as successful (up to 60% of embryos transferred developed to term) as surgical methods yet was simpler and eliminated surgical trauma to the recipient mouse.